Shops, homes and historic palaces filled with water in Venice and authorities said 70 percent of the lagoon city was flooded.
High water in Venice reached 149 cm (5ft), the sixth highest level since records began in 1872, forcing residents to wade through waist-deep water. Tourists in swimming costumes sat at cafe tables under the water.
There was no immediate estimate of damage to the beautiful northeastern city.
In Tuscany, 23 centimetres (9 inches) of rain fell in four hours, causing the Ricortola and Parmignola rivers to flood, according to the regional government.
"It has been devastating," said Roberto Pucci, the mayor of Massa Carrara in Tuscany, one of the worst hit areas.
"I saw at least six bridges destroyed in the hills, floods, landslides, vineyards and olive groves swept away. If there hasn't been a death it's a miracle," he told Corriere della Sera newspaper.
Local media said dozens of people took refuge on their roofs after rivers burst their banks in central Italy.
Environment Minister Corrado Clini called for more funding to shore up Italy's weather defences. Bad weather with torrential rain was due to continue through Tuesday, forecasters said.
It was the fourth time since 2000 that Venice had been hit by record high water, and the city's environment officer said the latest flooding was the result of global climate change.
A barrier to protect the city from repeated winter flooding, which has been planned for decades, is due to be finished by 2015. (Reporting by Steve Scherer and Naomi O'Leary; editing by Barry Moody)
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